Nationals Roster & Staff
Transactions
| Date | Transaction |
| 05/31/25 | Washington Nationals designated RHP Jorge López for assignment. |
| 05/31/25 | Washington Nationals recalled RHP Eduardo Salazar from Rochester Red Wings. |
| 05/28/25 | Washington Nationals activated 1B Andrés Chaparro from the 10-day injured list. |
| 05/28/25 | Washington Nationals signed free agent CF Delino DeShields to a minor league contract. |
| 05/28/25 | Washington Nationals optioned 1B Andrés Chaparro to Rochester Red Wings. |
| 05/23/25 | Washington Nationals placed CF Jacob Young on the 10-day injured list retroactive to May 20, 2025. Left shoulder AC sprain. |
| 05/23/25 | Washington Nationals selected the contract of CF Daylen Lile from Rochester Red Wings. |
| 05/22/25 | Washington Nationals sent 1B Andrés Chaparro on a rehab assignment to Rochester Red Wings. |
| 05/22/25 | Washington Nationals sent 1B Andrés Chaparro on a rehab assignment to Rochester Red Wings. |
| 05/21/25 | Washington Nationals placed RF Dylan Crews on the 10-day injured list. Left oblique strain. |
| 05/21/25 | Washington Nationals recalled CF Robert Hassell III from Rochester Red Wings. |
| 05/16/25 | Washington Nationals activated 2B Luis García Jr. from the paternity list. |
| 05/16/25 | Washington Nationals optioned 3B Trey Lipscomb to Rochester Red Wings. |
| 05/14/25 | Washington Nationals sent 1B Andrés Chaparro on a rehab assignment to FCL Nationals. |
| 05/13/25 | Washington Nationals signed free agent LHP Tommy Kane to a minor league contract. |
| 05/13/25 | Washington Nationals signed free agent RHP Bryce Conley to a minor league contract. |
| 05/13/25 | Washington Nationals placed 2B Luis García Jr. on the paternity list. |
| 05/13/25 | Washington Nationals recalled 3B Trey Lipscomb from Rochester Red Wings. |
| 05/11/25 | Washington Nationals activated RHP Cade Cavalli from the 15-day injured list. |
| 05/11/25 | Washington Nationals optioned RHP Cade Cavalli to Rochester Red Wings. |
| 05/10/25 | Washington Nationals released RHP Lucas Sims. |
| 05/10/25 | Washington Nationals recalled RHP Zach Brzykcy from Rochester Red Wings. |
| 05/09/25 | Washington Nationals signed free agent RHP Euri Montero to a minor league contract. |
| 05/07/25 | Washington Nationals activated RHP Michael Soroka from the 15-day injured list. |
| 05/07/25 | Washington Nationals optioned RHP Zach Brzykcy to Rochester Red Wings. |
| 05/07/25 | Washington Nationals optioned RHP Eduardo Salazar to Rochester Red Wings. |
| 05/06/25 | Washington Nationals signed free agent RHP Parker Dunshee to a minor league contract. |
| 05/06/25 | Washington Nationals signed free agent RHP Adrian Sampson to a minor league contract. |
| 05/06/25 | Washington Nationals recalled RHP Zach Brzykcy from Rochester Red Wings. |
| 05/04/25 | Washington Nationals sent LHP Colin Poche outright to Rochester Red Wings. |
| 05/02/25 | Washington Nationals sent RHP Michael Soroka on a rehab assignment to Rochester Red Wings. |
| 05/01/25 | Washington Nationals designated LHP Colin Poche for assignment. |
| 05/01/25 | Washington Nationals signed free agent LHP Andrew Chafin. |