Nationals Roster & Staff
Transactions
| Date | Transaction |
| 06/28/16 | Washington Nationals selected the contract of Lucas Giolito from Harrisburg Senators. |
| 06/28/16 | Washington Nationals released RHP Taylor Jordan. |
| 06/28/16 | Washington Nationals optioned RHP Rafael Martin to Syracuse Chiefs. |
| 06/27/16 | Washington Nationals sent RHP Jonathan Papelbon on a rehab assignment to Potomac Nationals. |
| 06/27/16 | Washington Nationals signed free agent RHP Whit Mayberry to a minor league contract. |
| 06/27/16 | Washington Nationals recalled Rafael Martin from Syracuse Chiefs. |
| 06/26/16 | Washington Nationals placed RHP Stephen Strasburg on the 15-day disabled list retroactive to June 16, 2016. Upper back strain. |
| 06/23/16 | Washington Nationals signed SS Tyler Beckwith. |
| 06/23/16 | Washington Nationals signed LHP Ryan Williamson. |
| 06/22/16 | Washington Nationals signed RF Nick Banks. |
| 06/21/16 | Washington Nationals signed RHP Samuel Held. |
| 06/21/16 | Washington Nationals signed CF Armond Upshaw. |
| 06/20/16 | Washington Nationals signed 1B Conner Simonetti. |
| 06/20/16 | Washington Nationals signed RHP Sterling Sharp. |
| 06/20/16 | Washington Nationals signed RHP Michael Rishwain. |
| 06/20/16 | Washington Nationals signed RHP Joseph Baltrip. |
| 06/20/16 | Washington Nationals signed 2B Jake Noll. |
| 06/20/16 | Washington Nationals signed RHP A.J. Bogucki. |
| 06/20/16 | Washington Nationals signed LHP Jake Barnett. |
| 06/20/16 | Washington Nationals signed LHP Jesus Luzardo. |
| 06/20/16 | Washington Nationals signed 3B Sheldon Neuse. |
| 06/20/16 | Washington Nationals signed LHP Jonny Reid. |
| 06/20/16 | Washington Nationals signed C Joey Harris. |
| 06/20/16 | Washington Nationals signed RHP Kyle Simonds. |
| 06/20/16 | Washington Nationals signed RHP Phil Morse. |
| 06/20/16 | Washington Nationals signed LHP Ben Braymer. |
| 06/20/16 | Washington Nationals signed RHP Jacob Howell. |
| 06/20/16 | Washington Nationals signed SS Branden Boggetto. |
| 06/20/16 | Washington Nationals signed LHP Jeremy McDonald. |
| 06/20/16 | Washington Nationals signed C CJ Picerni. |
| 06/20/16 | Washington Nationals signed OF Jack Sundberg. |
| 06/17/16 | Washington Nationals signed OF Daniel Johnson. |
| 06/17/16 | Washington Nationals signed SS Paul Panaccione. |
| 06/17/16 | Washington Nationals signed C Tres Barrera. |
| 06/16/16 | Washington Nationals signed free agent C Chance Shepard to a minor league contract. |
| 06/16/16 | Washington Nationals signed SS Carter Kieboom. |
| 06/15/16 | Washington Nationals signed free agent RHP Hector Ramirez to a minor league contract. |
| 06/14/16 | Washington Nationals signed free agent RHP Rafael Gomez to a minor league contract. |
| 06/14/16 | Washington Nationals placed RHP Jonathan Papelbon on the 15-day disabled list retroactive to June 13, 2016. Right intercostal strain. |
| 06/14/16 | Washington Nationals activated RHP Matt Belisle from the 15-day disabled list. |
| 06/09/16 | Washington Nationals signed free agent RHP J.D. Martin to a minor league contract. |
| 06/08/16 | Washington Nationals sent RHP Matt Belisle on a rehab assignment to Syracuse Chiefs. |
| 06/07/16 | Washington Nationals signed free agent 2B Steve Lombardozzi to a minor league contract. |
| 06/06/16 | Washington Nationals activated 1B Ryan Zimmerman from the paternity list. |
| 06/06/16 | Washington Nationals signed free agent 2B Steve Lombardozzi to a minor league contract. |
| 06/06/16 | Washington Nationals optioned SS Trea Turner to Syracuse Chiefs. |
| 06/03/16 | Washington Nationals recalled Trea Turner from Syracuse Chiefs. |
| 06/03/16 | Washington Nationals placed 1B Ryan Zimmerman on the paternity list. |