Nationals Roster & Staff
Transactions
| Date | Transaction |
| 09/29/25 | Washington Nationals activated RHP Cole Henry from the 15-day injured list. |
| 09/29/25 | Washington Nationals activated C Keibert Ruiz from the 7-day injured list. |
| 09/29/25 | Washington Nationals activated LHP MacKenzie Gore from the 15-day injured list. |
| 09/29/25 | Washington Nationals activated RHP Mason Thompson from the 15-day injured list. |
| 09/29/25 | Washington Nationals recalled RHP Andry Lara from Rochester Red Wings. |
| 09/29/25 | Washington Nationals recalled 3B Trey Lipscomb from Rochester Red Wings. |
| 09/29/25 | Washington Nationals recalled C C.J. Stubbs from Rochester Red Wings. |
| 09/29/25 | Washington Nationals recalled RHP Eduardo Salazar from Rochester Red Wings. |
| 09/29/25 | Washington Nationals recalled 3B José Tena from Rochester Red Wings. |
| 09/29/25 | Washington Nationals recalled RHP Ryan Loutos from Rochester Red Wings. |
| 09/29/25 | Washington Nationals recalled LHP Jake Eder from Rochester Red Wings. |
| 09/29/25 | Washington Nationals recalled RHP Zach Brzykcy from Rochester Red Wings. |
| 09/24/25 | Washington Nationals signed free agent 2B Orelvis Martinez to a minor league contract. |
| 09/23/25 | Washington Nationals placed RHP Mason Thompson on the 15-day injured list retroactive to September 20, 2025. Right biceps tendinitis. |
| 09/23/25 | Washington Nationals recalled RHP Orlando Ribalta from Rochester Red Wings. |
| 09/23/25 | Washington Nationals recalled RHP Julian Fernández from Rochester Red Wings. |
| 09/19/25 | Washington Nationals sent RHP Josiah Gray on a rehab assignment to Rochester Red Wings. |
| 09/17/25 | Washington Nationals optioned RHP Orlando Ribalta to Rochester Red Wings. |
| 09/17/25 | Washington Nationals recalled RHP Sauryn Lao from Rochester Red Wings. |
| 09/14/25 | Washington Nationals placed RHP Cole Henry on the 15-day injured list. Back strain. |
| 09/14/25 | Washington Nationals recalled RHP Orlando Ribalta from Rochester Red Wings. |
| 09/13/25 | Washington Nationals sent RHP Josiah Gray on a rehab assignment to Harrisburg Senators. |
| 09/11/25 | Washington Nationals placed LHP MacKenzie Gore on the 15-day injured list. Right ankle impingement. |
| 09/11/25 | Washington Nationals activated LHP MacKenzie Gore from the 15-day injured list. |
| 09/11/25 | Washington Nationals optioned RHP Orlando Ribalta to Rochester Red Wings. |
| 09/07/25 | Washington Nationals sent RHP Josiah Gray on a rehab assignment to Wilmington Blue Rocks. |
| 09/07/25 | Washington Nationals sent RHP Josiah Gray on a rehab assignment to Wilmington Blue Rocks. |
| 09/05/25 | Washington Nationals sent 2B Darren Baker outright to Rochester Red Wings. |
| 09/03/25 | Washington Nationals designated 2B Darren Baker for assignment. |
| 09/03/25 | Washington Nationals optioned RHP Sauryn Lao to Rochester Red Wings. |
| 09/03/25 | Washington Nationals claimed RHP Sauryn Lao off waivers from Seattle Mariners. |
| 09/02/25 | Washington Nationals signed free agent C Jorge Alfaro. |
| 09/02/25 | Washington Nationals transferred C Drew Millas from the 10-day injured list to the 60-day injured list. Left second finger fracture and dislocation. |
| 09/02/25 | Washington Nationals sent C Keibert Ruiz on a rehab assignment to Harrisburg Senators. |
| 09/02/25 | Washington Nationals optioned C C.J. Stubbs to Rochester Red Wings. |
| 09/01/25 | Washington Nationals recalled SS Nasim Nuñez from Rochester Red Wings. |
| 09/01/25 | Washington Nationals selected the contract of LHP Andrew Alvarez from Rochester Red Wings. |